6KEL

Crystal structure of Drosophila melanogaster Noppera-bo, glutathione S-transferase epsilon 14 (DmGSTE14), in apo-form

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2015-12-09
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.10000
Spacegroup name	P 21 21 21
Unit cell lengths	58.730, 76.750, 104.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.640 - 1.400
R-factor	0.1569
Rwork	0.156
R-free	0.17990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4png
RMSD bond length	0.013
RMSD bond angle	1.265
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549, 1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.640	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.095	0.662
Rmeas	0.098	0.694
Number of reflections	91281	8464
<I/σ(I)>	23.2	3.31
Completeness [%]	96.0	91.4
Redundancy	13.6	11.2
CC(1/2)		0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	27.5% (w/v) PEG 3350, 100 mM MES-NaOH, and pH 5.4