6KE1
Crystal structure of TtCas1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 150.432, 150.432, 219.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.600 - 3.390 |
| R-factor | 0.2212 |
| Rwork | 0.221 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kdv |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.016 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.460 |
| High resolution limit [Å] | 3.390 | 3.400 |
| Number of reflections | 19271 | 953 |
| <I/σ(I)> | 28.8 | |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 8.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 1.8-2.3M NaCl, 0.1M Bis-Tris pH 5.5 |
