6KDL
Crystal structure of human DNMT3B-DNMT3L complex (I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2018-06-29 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 192.932, 192.932, 50.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.468 - 3.274 |
| R-factor | 0.1803 |
| Rwork | 0.179 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yx2 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.390 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Rmerge | 0.052 | 0.446 |
| Number of reflections | 28675 | 1335 |
| <I/σ(I)> | 17.61 | |
| Completeness [%] | 98.8 | |
| Redundancy | 2.7 | |
| CC(1/2) | 0.833 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 278 | 20 mM Tris-HCl (pH 7.4), 200 mM NaCl, 5% glycerol, and 0.5 mM TCEP |
