6KDK
HIV-1 reverse transcriptase with Q151M/Y115F/F116Y:DNA:dCTP ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-17 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1000 |
Spacegroup name | H 3 |
Unit cell lengths | 284.852, 284.852, 95.926 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.743 - 2.560 |
R-factor | 0.1922 |
Rwork | 0.190 |
R-free | 0.23420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xn1 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.805 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.560 | 2.560 |
Rmeas | 0.092 | |
Number of reflections | 93456 | 4627 |
<I/σ(I)> | 13 | 1.8 |
Completeness [%] | 100.0 | |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | BIS-TRIS-HCL, DI-AMMONIUM HYDROGEN CITRATE, MGCL2, PEG 6000, SUCROSE, GLYCEROL |