6KDH
Antibody 64M-5 Fab including isoAsp in ligand-free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-04 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 101.789, 150.485, 65.151 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.510 - 2.470 |
| Rwork | 0.194 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ehl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.550 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.071 | 0.365 |
| Number of reflections | 17039 | 1271 |
| <I/σ(I)> | 18.8 | 3.7 |
| Completeness [%] | 92.2 | 73.4 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 8% PEG3350, 8% isopropanol, 0.1 M sodium citrate |
