6KDD

endoglucanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	50.138, 54.895, 128.166
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.730 - 1.920
Rwork	0.201
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.990
High resolution limit [Å]	1.920	1.920
Rmeas	0.105	0.474
Rpim	0.034	0.149
Number of reflections	27732	2715
<I/σ(I)>	4	2.6
Completeness [%]	100.0	100
Redundancy	9.6	9.7
CC(1/2)	0.985	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG4000