6KDC

crystal structure of Fpglu1 from fervidobacterium pennivoraus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-07-01
Detector	MAC Science DIP-320
Wavelength(s)	0.989
Spacegroup name	I 2 2 2
Unit cell lengths	63.040, 101.397, 170.542
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.630 - 1.850
Rwork	0.154
R-free	0.18000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.990
High resolution limit [Å]	1.850	1.850
Rmeas	0.106	0.507
Rpim	0.028	0.132
Number of reflections	46813	2715
<I/σ(I)>	4	3.1
Completeness [%]	100.0
Redundancy	9.6	14.4
CC(1/2)		0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG4000