6KD2
Room temperature structure of glucose isomerase delivered in gelatin by serial millisecond crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 298.15 |
Detector technology | PIXEL |
Collection date | 2019-04-03 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.9796 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 94.520, 100.170, 103.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.003 - 1.700 |
R-factor | 0.1931 |
Rwork | 0.193 |
R-free | 0.22360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zye |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.400 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 54280 | 5364 |
<I/σ(I)> | 5.15 | 1.73 |
Completeness [%] | 100.0 | 100 |
Redundancy | 1488.7 | 1021.5 |
CC(1/2) | 0.982 | 0.446 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7 | 293.5 | Tris-HCl, Ammonium sulfate, Magnesium sulfate |