6KCS
Crystal structure of HIRAN domain of HLTF in complex with duplex DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-27 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 45.363, 45.363, 125.827 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.950 - 2.100 |
R-factor | 0.23038 |
Rwork | 0.229 |
R-free | 0.26406 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xzf |
RMSD bond length | 0.003 |
RMSD bond angle | 0.993 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.071 | 0.605 |
Number of reflections | 9284 | 1336 |
<I/σ(I)> | 11.9 | |
Completeness [%] | 99.7 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | PEG |