6KCQ
Crystal structure of yedK with ssDNA containing an abasic site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 |
Unit cell lengths | 70.671, 70.671, 44.423 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.610 - 1.701 |
R-factor | 0.1624 |
Rwork | 0.160 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kbs |
RMSD bond length | 0.007 |
RMSD bond angle | 0.885 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASES |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.081 | 0.409 |
Rpim | 0.027 | 0.220 |
Number of reflections | 24080 | 2271 |
<I/σ(I)> | 23 | |
Completeness [%] | 99.1 | 94.3 |
Redundancy | 8.4 | 3.7 |
CC(1/2) | 0.999 | 0.444 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293 | 0.05M calcium chloride dihydrate 0.1M Bis-Tris pH6.5 30% MPEG550 |