Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.666, 76.326, 85.256 |
| Unit cell angles | 101.75, 106.08, 113.75 |
Refinement procedure
| Resolution | 29.940 - 2.400 |
| R-factor | 0.1997 |
| Rwork | 0.198 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kd6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.571 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 0.234 |
| Number of reflections | 55088 | 5126 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 96.3 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.1M Tris-HCl, pH 8.5and 25% PEG 3350 |
