6KCJ

Crystal structure of H5N2 hemagglutinin Apo-Q226L mutant from A/chicken/Taiwan/0502/2012

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	110.439, 110.439, 314.139
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.152 - 2.460
R-factor	0.206
Rwork	0.203
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ykc
RMSD bond length	0.008
RMSD bond angle	1.049
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.550
High resolution limit [Å]	2.460	2.460
Rmerge	0.134	1.415
Rmeas	0.140	1.483
Rpim	0.042	0.441
Number of reflections	27258	2682
<I/σ(I)>	21.4	2
Completeness [%]	100.0	100
Redundancy	11.1
CC(1/2)	0.990	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Tris, 0.2M MgCl, 16%PEG8000