6KCB

Room temperature structure of lysozyme delivered in shortening A by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2019-04-03
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	79.450, 79.450, 38.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.180 - 1.800
R-factor	0.1783
Rwork	0.177
R-free	0.20530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ig6
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	11974	1148
<I/σ(I)>	7.87	2.21
Completeness [%]	100.0	100
Redundancy	1451.9	1011.7
CC(1/2)	0.994	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.5	293.5	sodium acetate, PEG 8000, NaCl