6KC2
Crystal Structure of Lectin from Pleurotus ostreatus in complex with Rhamnose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-16 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 152.402, 152.402, 146.035 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.649 - 2.246 |
| R-factor | 0.2036 |
| Rwork | 0.203 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 4.840 | 2.250 |
| Rmerge | 0.180 | 0.046 | 1.669 |
| Rmeas | 0.185 | 0.048 | 1.716 |
| Rpim | 0.042 | 0.011 | 0.396 |
| Total number of observations | 908329 | ||
| Number of reflections | 47965 | 5122 | 4693 |
| <I/σ(I)> | 3.4 | ||
| Completeness [%] | 99.7 | 99.4 | 100 |
| Redundancy | 18.9 | 19 | 18.4 |
| CC(1/2) | 0.999 | 0.865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 300 | 0.9M Na2HPO4/K2HPO4, 33% glycerol |
