6KBY

Crystal structure of a class C beta lactamase in complex with AMP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-26
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.000000
Spacegroup name	C 1 2 1
Unit cell lengths	132.270, 60.230, 56.140
Unit cell angles	90.00, 112.04, 90.00

Refinement procedure

Resolution	41.036 - 1.097
R-factor	0.169
Rwork	0.169
R-free	0.18120
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.036	1.102
High resolution limit [Å]	1.097	1.097
Rmerge	0.058
Rmeas	0.063
Rpim	0.024
Number of reflections	165696	12228
<I/σ(I)>	13.21
Completeness [%]	99.3
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		295	0.1M sodium cacodylate pH 5.5, 0.2M sodium chloride, 35% polyethylene glycol 400