6KBM
Crystal structure of Vac8 bound to Atg13
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.727, 92.391, 139.879 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.841 - 2.902 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.24590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xjg |
RMSD bond length | 0.004 |
RMSD bond angle | 0.824 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.120 | 0.435 |
Number of reflections | 17745 | 794 |
<I/σ(I)> | 11.27 | |
Completeness [%] | 95.2 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 400, Tris, Ethylene glycol, PEG 3350, DTT |