6KBD
fused To-MtbCsm1 with 2dATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.801, 53.317, 124.134 |
| Unit cell angles | 90.00, 97.78, 90.00 |
Refinement procedure
| Resolution | 41.000 - 3.000 |
| Rwork | 0.237 |
| R-free | 0.28350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uw2 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmeas | 0.222 | 0.636 |
| Rpim | 0.092 | 0.283 |
| Number of reflections | 15522 | 759 |
| <I/σ(I)> | 23.6 | |
| Completeness [%] | 98.4 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.855 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M BICINE pH 8.7 and 15% PEG 1,500 |
