6KAK

Crystal structure of FKRP in complex with Mg ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-29
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	76.822, 119.419, 258.075
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.406 - 2.056
R-factor	0.1979
Rwork	0.196
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.724
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.406	2.079
High resolution limit [Å]	2.056	2.056
Number of reflections	147651	4318
<I/σ(I)>	18.68
Completeness [%]	100.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	4-8% PEG 4,000, 0.1 M HEPES-NaOH (pH7.5), and 0.2 M MgCl2