6KA7

The complex structure of Human IgG Fc and its binding Repebody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2015-12-12
Detector	OXFORD ONYX CCD
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	59.926, 107.420, 171.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.235 - 3.000
R-factor	0.2611
Rwork	0.257
R-free	0.33310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.246
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.050
High resolution limit [Å]	3.000	8.090	3.000
Rmerge	0.249	0.085
Total number of observations	316090
Number of reflections	23908	1329	1199
<I/σ(I)>	4.4
Completeness [%]	100.0	99.8	100
Redundancy	13.2	10.3	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris HCl, PEG 4000, Sodium chloride