6K97
Crystal structure of fusion DH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-11 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.632, 66.564, 63.013 |
| Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
| Resolution | 48.490 - 2.500 |
| R-factor | 0.19722 |
| Rwork | 0.195 |
| R-free | 0.23501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ln9 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.676 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.049 | 0.600 |
| Number of reflections | 22153 | 2463 |
| <I/σ(I)> | 14.6 | 2.2 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | MES-Na, PEG monomethylether 5000, ammonium sulfate |
