6K95
Crystal structural of human glutathione-specific gamma-glutamylcyclotransferase 2 (ChaC2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.90000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 108.842, 62.161, 103.625 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 37.410 - 2.290 |
R-factor | 0.2405 |
Rwork | 0.239 |
R-free | 0.26840 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.288 | 2.300 |
Number of reflections | 31131 | |
<I/σ(I)> | 23.006 | |
Completeness [%] | 99.5 | |
Redundancy | 5.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 14%(w/v) polyethylene-glycol 8,000 (PEG 8000), 14% PEG, 100mM HEPES (pH 7.5) |