6K94

Crystal structure of the type III effector XopAI from Xanthomonas axonopodis pv. citri - a 70 residue N-terminal truncation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	110
Detector technology	CCD
Collection date	2018-04-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	62.780, 98.760, 77.450
Unit cell angles	90.00, 91.21, 90.00

Refinement procedure

Resolution	31.527 - 2.260
R-factor	0.1915
Rwork	0.189
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eln
Data reduction software	iMOSFLM (v7.2.2)
Data scaling software	pointless (v1.11.21)
Phasing software	PHASER (v2.8.0)
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.530	2.330
High resolution limit [Å]	2.260	2.260
Rmerge	0.142	0.783
Rmeas	0.167	0.922
Rpim	0.088	0.482
Number of reflections	44030	4034
<I/σ(I)>	7.5
Completeness [%]	99.4	99.3
Redundancy	3.5	3.4
CC(1/2)	0.990	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	10% (v/v) 2-propanol, 20% (w/v) PEG400, and 100 mM HEPES-NaOH, pH 9.5