6K94
Crystal structure of the type III effector XopAI from Xanthomonas axonopodis pv. citri - a 70 residue N-terminal truncation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2018-04-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.780, 98.760, 77.450 |
Unit cell angles | 90.00, 91.21, 90.00 |
Refinement procedure
Resolution | 31.527 - 2.260 |
R-factor | 0.1915 |
Rwork | 0.189 |
R-free | 0.23190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4eln |
Data reduction software | iMOSFLM (v7.2.2) |
Data scaling software | pointless (v1.11.21) |
Phasing software | PHASER (v2.8.0) |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.530 | 2.330 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.142 | 0.783 |
Rmeas | 0.167 | 0.922 |
Rpim | 0.088 | 0.482 |
Number of reflections | 44030 | 4034 |
<I/σ(I)> | 7.5 | |
Completeness [%] | 99.4 | 99.3 |
Redundancy | 3.5 | 3.4 |
CC(1/2) | 0.990 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 10% (v/v) 2-propanol, 20% (w/v) PEG400, and 100 mM HEPES-NaOH, pH 9.5 |