6K92
Pyridoxal Kinase from Leishmania donovani in complex with ADP and Ginkgotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-05-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.740, 71.520, 76.670 |
| Unit cell angles | 90.00, 99.27, 90.00 |
Refinement procedure
| Resolution | 35.570 - 1.850 |
| R-factor | 0.18085 |
| Rwork | 0.179 |
| R-free | 0.21425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.645 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.760 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 47360 | 2821 |
| <I/σ(I)> | 14.6 | |
| Completeness [%] | 95.1 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 16% PEG 8000, 0.1M Sodium Cacodylate, 0.32M Calcium Acetate, 6% Glycerol |
