6K8Z
Pyridoxal Kinase from Leishmania donovani in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-02-23 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.630, 71.080, 77.310 |
| Unit cell angles | 90.00, 98.93, 90.00 |
Refinement procedure
| Resolution | 42.980 - 1.900 |
| R-factor | 0.17488 |
| Rwork | 0.173 |
| R-free | 0.21703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lhp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.564 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.980 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 44763 | 2822 |
| <I/σ(I)> | 14 | |
| Completeness [%] | 97.2 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 18% PEG 8000, 0.1M Sodium Cacodylate, 0.32M Calcium Acetate, 4% Glycerol |
