6K8E

Global regulatory element SarX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97774
Spacegroup name	P 41
Unit cell lengths	33.405, 33.405, 231.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.405 - 2.002
R-factor	0.2193
Rwork	0.217
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hs5
RMSD bond length	0.007
RMSD bond angle	0.976
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.127	0.597
Number of reflections	17045	1745
<I/σ(I)>	19.26	6.9
Completeness [%]	100.0	99.99
Redundancy	13.3	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	0.1M Bicine pH 9.0, 2% 1,4-Dioxane, 10% PEG 20000