6K8E
Global regulatory element SarX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97774 |
| Spacegroup name | P 41 |
| Unit cell lengths | 33.405, 33.405, 231.006 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.405 - 2.002 |
| R-factor | 0.2193 |
| Rwork | 0.217 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hs5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.976 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.127 | 0.597 |
| Number of reflections | 17045 | 1745 |
| <I/σ(I)> | 19.26 | 6.9 |
| Completeness [%] | 100.0 | 99.99 |
| Redundancy | 13.3 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 0.1M Bicine pH 9.0, 2% 1,4-Dioxane, 10% PEG 20000 |
