6K8B
The open state of RGLG1 VWA domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97889 |
Spacegroup name | P 41 |
Unit cell lengths | 136.180, 136.180, 56.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.961 - 2.210 |
R-factor | 0.1681 |
Rwork | 0.167 |
R-free | 0.19000 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.140 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.962 | 2.270 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.150 | 1.106 |
Number of reflections | 52083 | 3843 |
<I/σ(I)> | 2 | |
Completeness [%] | 99.0 | 99 |
Redundancy | 13.7 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M magnesium chloride , 0.1 Msodium chloride, 10%(w/v) PEG1500, 5%(w/v) ethanol |