6K88

RGLG1 MIDAS binds calcium ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97913
Spacegroup name	P 21 21 21
Unit cell lengths	42.876, 70.727, 146.403
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.997 - 1.788
R-factor	0.1955
Rwork	0.193
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.101
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.173	0.146	1.287
Rmeas	0.181	0.152	1.349
Rpim	0.052	0.043	0.394
Total number of observations	498505
Number of reflections	42755	4578	4210
<I/σ(I)>	7.6
Completeness [%]	99.6	99.8	99.7
Redundancy	11.7	12	11.4
CC(1/2)		0.988	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M CaCl2, 0.1 M Tris-HCl ph 8.0, 20%PEG4k