6K82

RGLG1 mutant-D338A E378A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.978897
Spacegroup name	P 21 21 21
Unit cell lengths	44.454, 73.327, 82.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.140 - 1.402
R-factor	0.196
Rwork	0.195
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.086
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.420
High resolution limit [Å]	1.400	3.800	1.400
Rmerge	0.084	0.054	1.249
Rmeas	0.088	0.056	1.393
Rpim	0.024	0.016	0.586
Total number of observations	571007
Number of reflections	48695	2905	1303
<I/σ(I)>	3.6
Completeness [%]	91.0	100	49.6
Redundancy	11.7	12.5	4.5
CC(1/2)		0.999	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M NaAc, 0.1 M sodium cacodylate ph 6.5, 30% PEG8K