6K7Z
Crystal structure of a GH18 chitinase from Pseudoalteromonas aurantia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 68.618, 87.486, 88.836 |
| Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
| Resolution | 33.019 - 1.799 |
| R-factor | 0.2133 |
| Rwork | 0.212 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gsj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.843 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.863 |
| High resolution limit [Å] | 1.799 | 1.799 |
| Rmerge | 0.128 | 0.316 |
| Number of reflections | 96262 | 9086 |
| <I/σ(I)> | 30.3 | 10.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% (w/v) PEG 8000, 0.05 M KH2PO4 |
