6K7U
Crystal structure of beta-2 microglobulin (beta2m) of Bat (Pteropus Alecto)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | OSCILLATION CAMERA |
Collection date | 2017-05-03 |
Detector | SDMS |
Wavelength(s) | 0.97776 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 33.707, 114.641, 57.307 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.527 - 1.601 |
R-factor | 0.1958 |
Rwork | 0.195 |
R-free | 0.20220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.015 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 15045 | 15045 |
<I/σ(I)> | 28.036 | |
Completeness [%] | 100.0 | |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M BIS-TRIS pH 6.5, 8% w/v Polyethylene glycol monomethyl ether 5000 |