6K7D
Crystal structure of MBPapo-Tim21 fusion protein with a 16-residue helical linker
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-04 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.010, 88.840, 119.364 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.631 - 2.000 |
R-factor | 0.1781 |
Rwork | 0.175 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1peb 2ciu |
RMSD bond length | 0.007 |
RMSD bond angle | 1.097 |
Data reduction software | HKL-2000 (714) |
Data scaling software | HKL-2000 (714) |
Phasing software | PHENIX (1.8_1069) |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.118 | |
Number of reflections | 34759 | 1709 |
<I/σ(I)> | 10.03 | 2.84 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.3 | 6.3 |
CC(1/2) | 0.736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MMT, pH 6.0, 24% PEG 1500 (w/v), 0.01M Betaine hydrochloride (additive) |