6K77
Crystal structure of AMPPNP bound Ck1a alpha from C. neoformans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-27 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.97 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.283, 95.853, 93.396 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.927 - 2.400 |
R-factor | 0.2225 |
Rwork | 0.216 |
R-free | 0.28220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lp4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.064 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.930 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.186 | 1.172 |
Number of reflections | 16004 | 1550 |
<I/σ(I)> | 10.02 | |
Completeness [%] | 99.7 | |
Redundancy | 13.5 | |
CC(1/2) | 0.996 | 0.913 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 0.2M lithium sulfate, 0.1M Tris pH 8.5, 30%(w/v) PEG 4000, 30%(w/v) dextran sulfate sodium salt, 5mM AMPPNP, 5mM MgCl2 |