6K76
Glycerol kinase form Thermococcus kodakarensis, complex structure with substrate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-20 |
Detector | AGILENT ATLAS CCD |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 218.862, 218.862, 66.244 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.361 - 3.050 |
R-factor | 0.1886 |
Rwork | 0.185 |
R-free | 0.26060 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.267 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((dev_3071: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.160 |
High resolution limit [Å] | 3.049 | 3.050 |
Number of reflections | 22560 | 2237 |
<I/σ(I)> | 23.49 | |
Completeness [%] | 99.9 | |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | imidazole, PEG1000, Calcium acetate, glycerol, AMP-PCP |