6K6W
Structure of RNase J2 from Staphylococcus epidermidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-11-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.550, 131.040, 115.900 |
| Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
| Resolution | 36.390 - 2.700 |
| R-factor | 0.2028 |
| Rwork | 0.200 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.863 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.390 | 2.700 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 47771 | 3949 |
| <I/σ(I)> | 9.5 | 3 |
| Completeness [%] | 90.6 | 81.8 |
| Redundancy | 5.6 | |
| CC(1/2) | 0.986 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 291 | 100mM tri-sodium citrate dihydrate pH 5.6, 20% PEG 4000, 20% Isopropanol, 40% Formamide |
