6K6U

Crystal structure of the human YTHDC2 YTH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 43
Unit cell lengths	49.520, 49.520, 119.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.069 - 2.270
R-factor	0.1978
Rwork	0.195
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.005
RMSD bond angle	0.769
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.070	2.440
High resolution limit [Å]	2.270	2.270
Number of reflections	13234	2472
<I/σ(I)>	9.3
Completeness [%]	99.9
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Tacsimate,Tris,PEG 3350