6K6R

Crystal structure of Saccharomyces cerevisiae single domain sulfurtranferase RDL2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	I 41
Unit cell lengths	79.753, 79.753, 110.382
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.595 - 2.471
Rwork	0.208
R-free	0.24720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d1p
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.510
High resolution limit [Å]	2.470	2.470
Number of reflections	11448
<I/σ(I)>	24.69
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289.15	3.5M Ammonium citrate