6K6I
The crystal structure of light-driven cyanobacterial chloride importer from Mastigocladopsis repens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2017-07-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 61.685, 61.685, 240.466 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.200 - 1.900 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5itc |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.200 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 21720 | 997 |
<I/σ(I)> | 21.1 | |
Completeness [%] | 96.1 | |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 4.9 | 293 | Magnesium nitrate hexahydrate, Sodium citrate |