6K5U
Crystal structure of the myb domain of S. pombe Tbf1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 105.393, 105.393, 41.302 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.262 - 2.079 |
| R-factor | 0.1912 |
| Rwork | 0.189 |
| R-free | 0.22210 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.140 |
| High resolution limit [Å] | 2.079 | 2.100 |
| Rmerge | 0.185 | 0.873 |
| Rmeas | 0.189 | 0.893 |
| Rpim | 0.038 | 0.183 |
| Number of reflections | 14514 | 696 |
| <I/σ(I)> | 32.455 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.7 | 23.4 |
| CC(1/2) | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 16% PEG 8000, 40mM Potassium phosphate dibasic, 20% Glycerol |
