6K5U

Crystal structure of the myb domain of S. pombe Tbf1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97778
Spacegroup name	P 4 21 2
Unit cell lengths	105.393, 105.393, 41.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.262 - 2.079
R-factor	0.1912
Rwork	0.189
R-free	0.22210
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.140
High resolution limit [Å]	2.079	2.100
Rmerge	0.185	0.873
Rmeas	0.189	0.893
Rpim	0.038	0.183
Number of reflections	14514	696
<I/σ(I)>	32.455
Completeness [%]	100.0	100
Redundancy	24.7	23.4
CC(1/2)		0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277.15	16% PEG 8000, 40mM Potassium phosphate dibasic, 20% Glycerol