6K5S
Crystal structure of the Helical domain of S. pombe Tbf1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 |
Unit cell lengths | 38.498, 80.639, 77.367 |
Unit cell angles | 111.45, 94.64, 92.05 |
Refinement procedure
Resolution | 38.297 - 1.904 |
R-factor | 0.2237 |
Rwork | 0.221 |
R-free | 0.27480 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.089 | 0.767 |
Rmeas | 0.077 | |
Rpim | 0.818 | |
Number of reflections | 65194 | 6438 |
<I/σ(I)> | 23.68 | 2.432 |
Completeness [%] | 97.6 | 96.5 |
Redundancy | 7.6 | 7.4 |
CC(1/2) | 0.903 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277.15 | 0.2M Ammonium citrate tribasic Ph7.0, 20%PEG3350 |