6K5P

Structure of mosquito-larvicidal Binary toxin receptor, Cqm1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-10-03
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	59.181, 191.486, 205.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.828 - 1.805
R-factor	0.1851
Rwork	0.183
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wy1
RMSD bond angle	15.072
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER (PHASER-EP)
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.230	1.900
High resolution limit [Å]	1.805	1.805
Rmerge	0.072	0.532
Rmeas	0.090	0.621
Rpim	0.044	0.318
Number of reflections	201511	31009
<I/σ(I)>	14.4	2.8
Completeness [%]	93.9	99.9
Redundancy	7.1	7.3
CC(1/2)	0.998	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	5mM CoCl2, 5mM CdCl2, 5mM MgCl2, 5mM NiCl2, 0.1mM HEPES pH 7.5, 12% PEG 3350