6K5M
The crystal structure of a serotonin N-acetyltransferase from Oryza Sativa (Rice)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.893, 66.893, 82.692 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.058 - 1.793 |
| R-factor | 0.2256 |
| Rwork | 0.225 |
| R-free | 0.23420 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Rmerge | 0.098 | 0.857 |
| Number of reflections | 18235 | 1781 |
| <I/σ(I)> | 67.435 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.9 | 26.3 |
| CC(1/2) | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 2M MgCl2, 18% PEG8000, Tris-HCl, PH8.0 |
