6K4D
Ancestral luciferase AncLamp in complex with ATP and D-luciferin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-29 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 48.760, 123.680, 177.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.888 - 1.700 |
R-factor | 0.1578 |
Rwork | 0.156 |
R-free | 0.18830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d1r |
RMSD bond length | 0.008 |
RMSD bond angle | 0.943 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.014 | 0.030 |
Number of reflections | 55829 | 2520 |
<I/σ(I)> | 75.6 | |
Completeness [%] | 96.3 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M trisodium citrate buffer (pH 5.5), 20% (w/v) PEG 3000 |