6K4C
Ancestral luciferase AncLamp in complex with DLSA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 48.310, 123.360, 177.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.967 - 2.100 |
R-factor | 0.2097 |
Rwork | 0.208 |
R-free | 0.24420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d1r |
RMSD bond length | 0.007 |
RMSD bond angle | 0.980 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.032 | 0.073 |
Number of reflections | 31197 | 3317 |
<I/σ(I)> | 14 | |
Completeness [%] | 99.7 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M trisodium citrate buffer (pH 5.5) , 20% (w/v) PEG 3000 |