6K3F
Crystal Structure of beta-Arrestin 2 in Complex with CXCR7 Phosphopeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-03 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.170, 127.910, 206.040 |
Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
Resolution | 46.804 - 2.300 |
R-factor | 0.2358 |
Rwork | 0.233 |
R-free | 0.24610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5w0p |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.804 | 2.070 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 162425 | 49820 |
<I/σ(I)> | 1.31 | |
Completeness [%] | 99.7 | |
Redundancy | 1 | |
CC(1/2) | 0.711 | 0.261 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M Ammonium acetate, 0.1M Bis-Tris pH5.5, 25% (w/v) PEG 3350 |