6K3A
Crystal structure of human PCNA in complex with DNMT1 PIP box motif.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.540, 81.598, 68.105 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 40.840 - 2.300 |
R-factor | 0.2195 |
Rwork | 0.217 |
R-free | 0.26430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mlo |
RMSD bond length | 0.005 |
RMSD bond angle | 0.852 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.840 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.062 | 0.664 |
Number of reflections | 34458 | 3366 |
<I/σ(I)> | 0 | 1.5 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.2 | 3.2 |
CC(1/2) | 0.997 | 0.705 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 1.6M sodium citrate |