6K35
Crystal structure of GH20 exo beta-N-acetylglucosaminidase from Vibrio harveyi in complex with NAG-thiazoline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.340, 145.040, 88.680 |
| Unit cell angles | 90.00, 109.66, 90.00 |
Refinement procedure
| Resolution | 29.350 - 2.360 |
| R-factor | 0.1972 |
| Rwork | 0.194 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rcn |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.419 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2-1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.350 | 2.440 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Number of reflections | 60694 | 6032 |
| <I/σ(I)> | 6.09 | |
| Completeness [%] | 99.6 | |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M Sodium Fluoride, 0.1M Bis Tris Propane pH 6.5, 20% PEG3350 |
