6K2W

Crystal structure of proteinase K from Engyodontium album

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	300
Detector technology	CCD
Collection date	2019-01-30
Detector	MPCCD
Wavelength(s)	1.24
Spacegroup name	P 43 21 2
Unit cell lengths	68.300, 68.300, 108.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.566 - 1.700
R-factor	0.1611
Rwork	0.160
R-free	0.18270
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	0.971
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.600	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	28952	1413
<I/σ(I)>	5.9
Completeness [%]	100.0
Redundancy	167

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.5	293	0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES