6K2M

Crystal structure of the complex of Proliferating Cell Nuclear Antigen from Leishmania donovani with arginine at 3.19 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 21 21 21
Unit cell lengths	135.926, 150.588, 171.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.630 - 3.190
R-factor	0.17011
Rwork	0.167
R-free	0.22695
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j0j
RMSD bond length	0.008
RMSD bond angle	1.727
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.630	3.240
High resolution limit [Å]	3.190	3.190
Rmerge	0.053	0.653
Rmeas	0.060	0.732
Rpim	0.028	0.323
Number of reflections	58894	2924
<I/σ(I)>	16.3	2.2
Completeness [%]	99.4	99.8
Redundancy	4.6	5
CC(1/2)	0.999	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3350, sodium malonate