6K2L
Crystal structure of the Siderophore-interacting protein SipS from Aeromonas hydrophila
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-10-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979463 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 130.402, 149.991, 55.896 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.933 - 2.500 |
R-factor | 0.2076 |
Rwork | 0.203 |
R-free | 0.26050 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.071 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 24604 | 176 |
<I/σ(I)> | 14.596 | 2.386 |
Completeness [%] | 99.3 | 97.7 |
Redundancy | 9.2 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 287 | 0.18 M Lithium sulfate monohydrate, 30% (w/v) Polyethylene glycol 4,000 and 0.1 M Tris hydrochloride, pH 8.5 |