6K2J

Crystal Structure of the DNA Complex of C. crescentus GapR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 65
Unit cell lengths	65.310, 65.310, 274.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.098 - 2.400
R-factor	0.2242
Rwork	0.221
R-free	0.26800
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.677
Data reduction software	HKL-2000 (7-16)
Data scaling software	HKL-2000 (7-16)
Phasing software	PHENIX ((1.11.1_2575: ???))
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.100	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.074	0.010
Number of reflections	25730	24904
<I/σ(I)>	40
Completeness [%]	87.7
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.005M Manganese(II) chloride tetrahydrate 0.05M Sodium citrate PH 5.6 1.25M 1,6-Hexanediol